Study the effect of vitamin D on monosodium glutamate induced testicular and ovarian toxicity in pubertal male and female albino rats
Author(s): Nehal M Gabr, Abeer Abed Abo Zeid, Nahid M Tahoon, Manar Elnady and Maessa Mohamed Elnhas
Abstract: Background and Aim:Monosodium glutamate (MSG) a commonly used food additive, has been reported to have adverse effects on reproductive organs. Vitamin D, known for its role in its various physiological functions, including reproductive health, was hypothesized to mitigate the toxic effects of MSG on reproductive organs. The aim of this work was to evaluate the toxic effect of MSG on testicular and ovarian tissue in pubertal male and female albino rats and the possible protective effect of vitamin D.
Material and Methods: A total of 80 pubertal albino local strain rats were divided into eight groups: four groups for males and four groups for females (10 rats) in each group control, vitamin D, MSG, MSG + vitamin D. The rats were administered MSG orally at a dose of 60 mg /kg/day dissolved in 0.5 ml distilled water for 30 consecutive days. Vitamin D was administered orally at a dose of 200 IU/kg /day for the same duration. 0.5 ml distilled water was administered to control groups.
Results: MSG administration caused degeneration, necrosis, disruption of normal architecture, multiple apoptotic cells shown by immunohistochemical examination of testicular caspase-3, DNA damage, and decreased TAC in testicular and ovarian tissue. Changes in male and female rats included increased body weight, decreased kisspeptin, serum GnRH, FSH, LH, testosterone, estradiol, and elevated TNFα levels. Vitamin D and MSG co-administration significantly reduced histopathological and hormonal alterations.
Conclusions: Vitamin D supplementation may have a role against MSG-induced ovarian and testicular toxicity in pubertal male and female albino rats.
DOI: 10.22271/27069567.2024.v6.i2a.548Pages: 12-24 | Views: 194 | Downloads: 107Download Full Article: Click Here
How to cite this article:
Nehal M Gabr, Abeer Abed Abo Zeid, Nahid M Tahoon, Manar Elnady, Maessa Mohamed Elnhas.
Study the effect of vitamin D on monosodium glutamate induced testicular and ovarian toxicity in pubertal male and female albino rats. Int J Adv Res Med 2024;6(2):12-24. DOI:
10.22271/27069567.2024.v6.i2a.548
